Experimental Autoimmune Uveitis: An Intraocular Inflammatory Mouse Model

Experimental Autoimmune Uveitis (EAU) is driven by immune cells responding to self-antigens. Many features of this non-infectious, intraocular inflammatory disease model recapitulate the clinical phenotype posterior uveitis affecting humans. EAU has been used reliably study efficacy novel therapeutics, their mode action and further investigate mechanisms that underpin progression disorders. Here, we provide a detailed protocol on induction in C57BL/6J mouse - most widely organism with susceptibility disease. Clinical assessment severity will be demonstrated using fundoscopy, histological examination fluorescein angiography. The procedure involves subcutaneous injection an emulsion containing peptide (IRBP1-20) from ocular protein interphotoreceptor retinoid binding (also known as retinol 3), Complete Freund's Adjuvant (CFA) supplemented killed Mycobacterium tuberculosis. Injection viscous back neck followed single intraperitoneal Bordetella pertussis toxin. At onset symptoms (day 12-14) under general anesthesia, fundoscopic images are taken assess through examination. These data can directly compared those at later timepoints peak 20-22) differences analyzed. same time, allows investigator potential vessel permeability damage induced other strains both wildtype or genetically modified combined therapies offering flexibility for studying drug and/or mechanisms.